UPTAKE OF MAMMALIAN CHROMOSOMES BY MAMMALIAN CELLS

Uptake of Mammalian Chromosomes by Mammalian Cells

Chromosomes isolated from mouse leukemia L1210 cells were taken up by mouse macrophages, HeLa cells, and rat embryo fibroblasts following simple exposure in vitro. The process, which resembles pinocytosis or phagocytosis, was traced by autoradiography of chromosomes prelabeled with thymidine-H(3), and by staining techniques and phase contrast microscopy. During the first six hours, the uptake o...

Mammalian Chromosomes in Vitro

The complete DNA replication sequence of the entire complement of chromosomes in the Chinese hamster may be studied by using the method of continuous H(3)-thymidine labeling and the method of 5-fluorodeoxyuridine block with H(3)-thymidine pulse labeling as relief. Many chromosomes start DNA synthesis simultaneously at multiple sites, but the sex chromosomes (the Y and the long arm of the X) beg...

Engineering mammalian chromosomes.

Construction of a mammalian artificial chromosome (MAC) will develop our understanding of the requirements for normal chromosome maintenance, replication and segregation while offering the capacity for introducing genes into cells. Construction of MACs with telomere, centromere and replication function has been approached by two methods. The 'top down' strategy uses artificially induced chromos...

Uptake and release of carbon nanotubes by mammalian cells

Uptake of polynucleotides by intact mammalian cells (IV): synthetic homoribopolynucleotides.

säure und CHJ3 mit 52 bzw. 44% der eingesetzten spe­ zifischen Radioaktivität. Das bedeutet, daß sich die Mar­ kierungen beider Bruchstücke ungefähr wie 1:1 verhal­ ten, während sie bei einer Biosynthese des Methylisopelletierins aus 4 Acetateinheiten im Verhältnis 3:1 stehen sollten. Im Methioninversuch (Versuch 6) erwies sich Isopelletierin (1) als radioaktivitätsfrei. Die mar­ kierten Alkalo...